By Maria Slater
Secondly, to my knowledge Oct3/4 is a protein characteristic for pluripotent cells and such are not present in regenerating muscle. Reviewer 2 ReportThe authors demonstrated that the SIRT3 activation could have beneficial effects on microgravity-induced muscle tissue damage. To my opinion, the introduction can be reduced to one page focusing on the key effects of the microgravity to muscle cells. To my opinion, the introduction can be reduced to one page focusing on the key effects of the microgravity to muscle cells. A: We have shortened the Introduction that is now little bit over 1 page focusing mostly on muscle tissue and microgravity.
Abstract Two DNA polymerase isoenzymes, called DpA and DpB on the basis of their elution order from DEAE cellulose, were purified to homogeneity from the thermo-acidophilic eubacterium Bacillus acidocaldarius. The enzymes are weakly acidophilic proteins constituted by a single subunit of 117 and 103 kDa respectively. DpA and DpB differ in thermostability, in thermophilicity, in sensitivity to assay conditions and in resistance to sulphydryl-group blocking agents such as N-ethylmaleimide and p-hydroxymercuriobenzoate. They differ also in synthetic template-primer utilization, in the apparent Km for dNTPs and in processivity. In addition, DpA presents an exonuclease activity that preferentially hydrolyses DNA in the 5'-3' direction, whereas DpB lacks this activity.
